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Rabbit Anti-Heme Oxygenase Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-Heme Oxygenase Polyclonal Antibody AP52105
    293T cell lysate probed (AP52105) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody at 1:5000 90min in 37˚C.
  • 14 - Rabbit Anti-Heme Oxygenase Polyclonal Antibody AP52105
    Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated AP52105 at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession P09601
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Additional info
Gene ID 3162
Other Names HO-1; HSP32; HMOX1D; bK286B1; Heme oxygenase 1; HMOX1; HO; HO1
Dilution WB~~1/100~1/500
IHC-P~~1/100~1/500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name HMOX1
Synonyms HO, HO1
Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Cellular Location Microsome. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level)
Research Areas

BACKGROUND

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

REFERENCES

Yoshida T.,et al.Eur. J. Biochem. 171:457-461(1988).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Dunham I.,et al.Nature 402:489-495(1999).
Keyse S.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:99-103(1989).
Shibahara S.,et al.Eur. J. Biochem. 179:557-563(1989).

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