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Rabbit Anti-CYP11A1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-CYP11A1 Polyclonal Antibody AP52081
    Lane 1: mouse kidney lysates Lane 2: human kidney lysates probed with Anti CYP11A1/P450SCC Polyclonal Antibody, Unconjugated (AP52081) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 53/57kD. Observed band size: 53kD.
  • 14 - Rabbit Anti-CYP11A1 Polyclonal Antibody AP52081
    Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti CYP11A1/P450SCC Polyclonal Antibody, Unconjugated (AP52081) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
  • 1 - Rabbit Anti-CYP11A1 Polyclonal Antibody AP52081
    Mouse placenta lysates probed with Rabbit Anti-CYP11A1 Polyclonal Antibody, Unconjugated (AP52081) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:500 for 90 min at 37˚C.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession P05108
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 60102 Da
Additional info
Gene ID 1583
Other Names CYP11A; CYPXIA1; P45SCC; Cholesterol side-chain cleavage enzyme, mitochondrial; Cholesterol desmolase; Cytochrome P45 11A1; Cytochrome P45(scc); CYP11A1
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name CYP11A1
Synonyms CYP11A
Function Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Cellular Location Mitochondrion membrane.
Research Areas

BACKGROUND

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

REFERENCES

Chung B.-C.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:8962-8966(1986).
Morohashi K.,et al.J. Biochem. 101:879-887(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:671-675(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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