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>   首页   >   产品   >   一抗   >   精选抗体   >   Rabbit Anti-Caspase 8 Polyclonal Antibody   

Rabbit Anti-Caspase 8 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-Caspase 8 Polyclonal Antibody AP52053
    L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (AP52053) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:3000 for 90 min at 37˚C. Predicted band 12kD. Observed band size:48kD.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession O89110
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 55357 Da
Additional info
Gene ID 12370
Other Names MACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8
Dilution WB~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name Casp8
Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Tissue Location Expressed in a wide variety of tissues. Highest expression in spleen, thymus, lung, liver and kidney Lower expression in heart, brain, testis and skeletal muscle
Research Areas

BACKGROUND

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

REFERENCES

Sakamaki K.,et al.Eur. J. Biochem. 253:399-405(1998).
Van de Craen M.,et al.J. Mol. Biol. 284:1017-1026(1998).
Kioschis P.,et al.Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
Milovic-Holm K.,et al.EMBO J. 26:391-401(2007).
Villen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).

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