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mTERF Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - mTERF Antibody AP51875
    All lanes : Anti-mTERF Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q99551
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 39 KDa
Additional info
Gene ID 7978
Other Names Transcription termination factor 1, mitochondrial, Mitochondrial transcription termination factor 1, mTERF, mTERF1, MTERF1, MTERF
Target/Specificity KLH conjugated synthetic peptide derived from human mTERF
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name MTERF1
Synonyms MTERF
Function Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.
Cellular Location Mitochondrion.
Research Areas

BACKGROUND

Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.

REFERENCES

Fernandez-Silva P.,et al.EMBO J. 16:1066-1079(1997).
Scherer S.W.,et al.Science 300:767-772(2003).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).

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