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>   首页   >   产品   >   一抗   >   癌症   >   Pyruvate Carboxylase Antibody   

Pyruvate Carboxylase Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Pyruvate Carboxylase Antibody AP51826
    Anti-Pyruvate Carboxylase Antibodyat 1:1000 dilution + Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P11498
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 130 KDa
Additional info
Gene ID 5091
Other Names Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB, PC
Target/Specificity KLH conjugated synthetic peptide derived from human Pyruvate Carboxylase
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PC
Function Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Cellular Location Mitochondrion matrix.
Research Areas

BACKGROUND

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.

REFERENCES

Wexler I.D.,et al.Biochim. Biophys. Acta 1227:46-52(1994).
Mackay N.,et al.Biochem. Biophys. Res. Commun. 202:1009-1014(1994).
Walker M.E.,et al.Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
Lamhonwah A.-M.,et al.Arch. Biochem. Biophys. 254:631-636(1987).
Freytag S.O.,et al.J. Biol. Chem. 259:12831-12837(1984).

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