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CD174 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CD174 Antibody AP51773
    Anti-CD174 Antibodyat 1:1000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P21217
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 42 KDa
Additional info
Gene ID 2525
Other Names Galactoside 3(4)-L-fucosyltransferase, Blood group Lewis alpha-4-fucosyltransferase, Lewis FT, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FUT3, FT3B, LE
Target/Specificity KLH conjugated synthetic peptide derived from human CD174
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name FUT3
Synonyms FT3B, LE
Function May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Cellular Location Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi
Tissue Location Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver
Research Areas

BACKGROUND

May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.

REFERENCES

Kukowska-Latallo J.F.,et al.Genes Dev. 4:1288-1303(1990).
Cameron H.S.,et al.J. Biol. Chem. 270:20112-20122(1995).
Rahim I.,et al.Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Matzhold E.M.,et al.Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).

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