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hnRNP E1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - hnRNP E1 Antibody AP51686
    Anti-hnRNP E1 Antibody at 1:1000 dilution + K562 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q15365
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 37 KDa
Additional info
Gene ID 5093
Other Names Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, hnRNP E1, Nucleic acid-binding protein SUB23, PCBP1
Target/Specificity KLH conjugated synthetic peptide derived from human hnRNP E1
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PCBP1
Function Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).
Cellular Location Nucleus. Cytoplasm. Note=Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm
Tissue Location Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands
Research Areas

BACKGROUND

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.

REFERENCES

Leffers H.,et al.Eur. J. Biochem. 230:447-453(1995).
Kiledjian M.,et al.EMBO J. 14:4357-4364(1995).
Aasheim H.-C.,et al.Nucleic Acids Res. 22:959-964(1994).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Olsen J.V.,et al.Cell 127:635-648(2006).

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