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Septin 7 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Septin 7 Antibody AP51501
    All lanes : Anti-Septin 7 Antibody at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q16181
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 51 KDa
Additional info
Gene ID 989
Other Names Septin-7, CDC10 protein homolog, SEPT7, CDC10
Target/Specificity KLH conjugated synthetic peptide derived from human Septin 7
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name SEPT7
Synonyms CDC10
Function Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.
Cellular Location Cytoplasm. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Cleavage furrow. Midbody. Cytoplasm, cytoskeleton, cilium axoneme. Note=Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase Associated with actin stress fibers (By similarity)
Tissue Location Widely expressed.
Research Areas

BACKGROUND

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.

REFERENCES

Nakatsuru S.,et al.Biochem. Biophys. Res. Commun. 202:82-87(1994).
Nagata K.,et al.J. Biol. Chem. 279:55895-55904(2004).
Hall P.A.,et al.J. Pathol. 206:269-278(2005).
Kremer B.E.,et al.Mol. Biol. Cell 16:4648-4659(2005).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).

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