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RCC2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RCC2 Antibody AP51470
    Anti-RCC2 Antibodyat 1:1000 dilution + Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9P258
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 56 KDa
Additional info
Gene ID 55920
Other Names Protein RCC2, RCC1-like protein TD-60, Telophase disk protein of 60 kDa, RCC2, KIAA1470, TD60
Target/Specificity KLH conjugated synthetic peptide derived from human RCC2
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name RCC2
Synonyms KIAA1470, TD60
Function Multifunctional protein that may effect its functions by regulating the activity of small GTPases, such as RAC1 and RALA (PubMed:12919680, PubMed:25074804, PubMed:26158537, PubMed:28869598). Required for normal progress through the cell cycle, both during interphase and during mitosis (PubMed:23388455, PubMed:12919680, PubMed:26158537). Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles (PubMed:12919680, PubMed:26158537). Required for normal organization of the microtubule cytoskeleton in interphase cells (PubMed:23388455). Functions as guanine nucleotide exchange factor (GEF) for RALA (PubMed:26158537). Interferes with the activation of RAC1 by guanine nucleotide exchange factors (PubMed:25074804). Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions (PubMed:25074804, PubMed:28869598). Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro) (PubMed:25074804, PubMed:28869598).
Cellular Location Nucleus, nucleolus. Nucleus. Cytoplasm, cytoskeleton. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle Chromosome. Midbody. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex (PubMed:12919680, PubMed:1939370, PubMed:7559776, PubMed:9914378). Colocalizes with cytoplasmic microtubules in interphase cells (PubMed:23388455) Colocalizes with RAC1 at the cell membrane (PubMed:25074804)
Research Areas

BACKGROUND

Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.

REFERENCES

Mollinari C.,et al.Dev. Cell 5:295-307(2003).
Nagase T.,et al.DNA Res. 7:143-150(2000).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Andreassen P.R.,et al.J. Cell Sci. 99:523-534(1991).
Martineau S.N.,et al.J. Cell Biol. 131:191-205(1995).

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