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Anti-Cyclin D1 antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - Anti-Cyclin D1 antibody AP50933
    Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (AP50933) at 1:200, followed by conjugation to the secondary antibody and DAB staining
  • 1 - Anti-Cyclin D1 antibody AP50933
    A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody AP50933 at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody at 1:5000 90min in 37˚C.
  • 4 - Anti-Cyclin D1 antibody AP50933
    RSC96 cells probed with Cyclin D1 Polyclonal Antibody, Unconjugated AP50933 at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P24385
Reactivity Human, Mouse, Rat, Dog
Host Rabbit
Clonality polyclonal
Calculated MW H=34; KDa
Additional info
Gene ID 595
Other Names G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
Dilution IHC-P~~1/100~1/500
WB~~1/100~1/500
FC~~1/20~1/100
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name CCND1
Synonyms BCL1, PRAD1
Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Cellular Location Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members
Research Areas
Elucidating the role of the FoxO3a transcription factor in the IGF-1-induced migration and invasion of uveal melanoma cancer cells.
Author : Yan F1,Liao R1,Farhan M2,Wang T3,Chen J3,Wang Z4,Little PJ5,Zheng W6.
Biomed Pharmacother. 2016 Dec;84:1538-1550. doi: 10.1016/j.biopha.2016.11.027. Epub 2016 Nov 20.
27881235

BACKGROUND

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

REFERENCES

Motokura T.,et al.Nature 350:512-515(1991).
Lew D.J.,et al.Cell 66:1197-1206(1991).
Xiong Y.,et al.Cell 65:691-699(1991).
Withers D.A.,et al.Mol. Cell. Biol. 11:4846-4853(1991).
Rimokh R.,et al.Blood 83:3689-3696(1994).

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