Anti-Cyclin D1 antibody
Purified Rabbit Polyclonal Antibody (Pab)
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Application ![]()
| WB |
---|---|
Primary Accession | P24385 |
Reactivity | Human, Mouse, Rat, Dog |
Host | Rabbit |
Clonality | polyclonal |
Calculated MW | H=34; KDa |
Gene ID | 595 |
---|---|
Other Names | G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1 |
Dilution | IHC-P~~1/100~1/500 WB~~1/100~1/500 FC~~1/20~1/100 |
Format | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce |
Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
Name | CCND1 |
---|---|
Synonyms | BCL1, PRAD1 |
Function | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
Cellular Location | Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members |
Author : Yan F1,Liao R1,Farhan M2,Wang T3,Chen J3,Wang Z4,Little PJ5,Zheng W6. Biomed Pharmacother. 2016 Dec;84:1538-1550. doi: 10.1016/j.biopha.2016.11.027. Epub 2016 Nov 20. |
27881235 |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
REFERENCES
Motokura T.,et al.Nature 350:512-515(1991).
Lew D.J.,et al.Cell 66:1197-1206(1991).
Xiong Y.,et al.Cell 65:691-699(1991).
Withers D.A.,et al.Mol. Cell. Biol. 11:4846-4853(1991).
Rimokh R.,et al.Blood 83:3689-3696(1994).

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