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PEDF antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - PEDF antibody AP50882
    Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-PEGF Polyclonal Antibody, Unconjugated (AP50882) at 1:200, followed by conjugation to the secondary antibody and DAB staining
  • 3 - PEDF antibody AP50882
    Formalin-fixed and paraffin embedded rat eye, ciliary body labeled Anti-PEGF Polyclonal Antibody, Unconjugated (AP50882) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.
  • 1 - PEDF antibody AP50882
    Western blot analysis of lysate from human plasma tissue lysate, using PEDF antibody(AP50882). AP50882 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysate at 20ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P36955
Reactivity Rat, Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 46kD; [uniprot,EPI]46; [RD]50; KDa
Additional info
Gene ID 5176
Other Names Pigment epithelium-derived factor, PEDF, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1, SERPINF1, PEDF
Dilution IHC-P~~1/100~1/500
IF~~1/100~1/200
WB~~1/1000
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name SERPINF1
Synonyms PEDF
Function Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Cellular Location Secreted. Melanosome. Note=Enriched in stage I melanosomes
Tissue Location Retinal pigment epithelial cells and blood plasma.
Research Areas

BACKGROUND

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

REFERENCES

Steele F.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:1526-1530(1993).
Tombran-Tink J.,et al.Mol. Vis. 2:11-11(1996).
Yin B.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Kim J.W.,et al.Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.

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