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AQP1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AQP1 Antibody AP50868
    Lane 1: mouse heart lysates Lane 2: mouse lung lysates probed with Anti AQP1/CHIP Polyclonal Antibody, Unconjugated AP50868 at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 28kD. Observed band size: 28kD.
  • 14 - AQP1 Antibody AP50868
    Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (AP50868) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
  • 14 - AQP1 Antibody AP50868
    Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated AP50868 at 1:200 overnight at 4°C, followed by a conjugated secondary antibody at [1:500] for 90 minutes and DAPI staining of the nuclei.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P29972
Reactivity Human, Mouse, Rat, Sheep, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 28; [uniprot]28,20,23,17; KDa
Additional info
Gene ID 358
Other Names Aquaporin-1, AQP-1, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, AQP1, CHIP28
Dilution WB~~1/100~1/500
IHC-P~~1/100~1/500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name AQP1
Synonyms CHIP28
Function Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Research Areas

BACKGROUND

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

REFERENCES

Preston G.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:11110-11114(1991).
Moon C.,et al.J. Biol. Chem. 268:15772-15778(1993).
Ruiz A.C.,et al.Biochim. Biophys. Acta 1282:174-178(1996).
Li X.,et al.Biochem. Mol. Biol. Int. 32:371-377(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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