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CSP Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CSP Antibody AP50867
    Western blot analysis of lysate from human brain tissue lysate,using CSP Antibody(AP50867). AP50867 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • 2 - CSP Antibody AP50867
    Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-CSP Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession Q9H3Z4
Reactivity Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep
Host Rabbit
Clonality Polyclonal
Calculated MW 22; [uniprot]22,19; [abcam]22; KDa
Additional info
Gene ID 80331
Other Names DnaJ homolog subfamily C member 5, Cysteine string protein, CSP, DNAJC5, CSP
Dilution WB~~ 1:500
IHC~~1:100-1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name DNAJC5
Synonyms CSP
Function May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).
Cellular Location Membrane; Lipid-anchor. Melanosome. Cell membrane. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart
Research Areas

BACKGROUND

May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).

REFERENCES

Coppola T.,et al.FEBS Lett. 391:269-272(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Hattori A.,et al.DNA Res. 7:357-366(2000).

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