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ACTG2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ACTG2 Antibody AP50866
    Western blot analysis of lysate from human heart tissue lysate,using ACTG2 Antibody(AP50866). AP50866 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • 2 - ACTG2 Antibody AP50866
    Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-ACTG2 Polyclonal Antibody, Unconjugated1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DABstaining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession P63267
Reactivity Human, Mouse, Rat, Chicken, Rabbit
Host Rabbit
Clonality Polyclonal
Calculated MW 42; [uniprot]42,37; [CST]45; KDa
Additional info
Gene ID 72
Other Names Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG
Dilution WB~~ 1:500
IHC~~1:100-1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name ACTG2
Synonyms ACTA3, ACTL3, ACTSG
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas

BACKGROUND

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

REFERENCES

Miwa T.,et al.Nucleic Acids Res. 18:4263-4263(1990).
Miwa T.,et al.Mol. Cell. Biol. 11:3296-3306(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).

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