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PACAP-27/38 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PACAP-27/38 Antibody AP50858
    Western blot analysis of lysate from human brain tissue lysate,using PACAP-27/38 Antibody(AP50858). AP50858 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • 2 - PACAP-27/38 Antibody AP50858
    Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-PACAP-27/38 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession P18509
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 19; [uniprot]19; [RD]19; KDa
Additional info
Gene ID 116
Other Names Pituitary adenylate cyclase-activating polypeptide, PACAP, PACAP-related peptide, PRP-48, Pituitary adenylate cyclase-activating polypeptide 27, PACAP-27, PACAP27, Pituitary adenylate cyclase-activating polypeptide 38, PACAP-38, PACAP38, ADCYAP1
Dilution WB~~1/500
IHC~~1/100-1/500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name ADCYAP1
Function Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.
Cellular Location Secreted.
Research Areas

BACKGROUND

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.

REFERENCES

Ohkubo S.,et al.DNA Cell Biol. 11:21-30(1992).
Hosoya M.,et al.Biochim. Biophys. Acta 1129:199-206(1992).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kimura C.,et al.Biochem. Biophys. Res. Commun. 166:81-89(1990).
Emery A.C.,et al.Sci. Signal. 6:RA51-RA51(2013).

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