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Tubulin Beta Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Tubulin Beta Antibody AP50855
    Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated (AP50855) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
  • 1 - Tubulin Beta Antibody AP50855
    Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated AP50855 at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
  • 14 - Tubulin Beta Antibody AP50855
    Formalin-fixed and paraffin embedded rat brain labeled with Anti-tubulin Beta Polyclonal Antibody, Unconjugated (AP50855) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • 1 - Tubulin Beta Antibody AP50855
    Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti tubulin Beta Polyclonal Antibody, Unconjugated (AP50855) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 50kD. Observed band size: 50kD.
  • 2 - Tubulin Beta Antibody AP50855
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-tubulin Beta Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession P07437
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 50 KDa
Additional info
Gene ID 203068
Other Names Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
Dilution WB~~ 1:500
IHC-P~~1/100~1/500
IHC~~1/100-1/500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name TUBB
Synonyms TUBB5
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research Areas
Single-walled carbon-nanohorns improve biocompatibility over nanotubes by triggering less protein-initiated pyroptosis and apoptosis in macrophages.
Author : He B1,2,3,Shi Y2,4,Liang Y1,2,3,Yang A1,2,3,Fan Z1,2,3,Yuan L5,Zou X5,Chang X5,Zhang H1,2,3,Wang X1,2,3,Dai W1,2,3,Wang Y1,2,3,Zhang Q6,7,8.
Nat Commun. 2018 Jun 19;9(1):2393. doi: 10.1038/s41467-018-04700-z.
29921862

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

REFERENCES

Lee M.G.-S.,et al.Cell 33:477-487(1983).
Hall J.L.,et al.Mol. Cell. Biol. 3:854-862(1983).
Crabtree D.V.,et al.Bioorg. Med. Chem. 9:1967-1976(2001).
Yu W.,et al.Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Shiina S.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.

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