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AZI1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AZI1 Antibody AP50845
    Western blot analysis of lysate from MCF-7 cell line,using AZI1 Antibody,was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • 2 - AZI1 Antibody AP50845
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using AZI1 antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession Q9UPN4
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 122117 Da
Additional info
Gene ID 22994
Other Names Centrosomal protein of 131 kDa, 5-azacytidine-induced protein 1, Pre-acrosome localization protein 1, CEP131, AZI1, KIAA1118
Dilution WB~~ 1:1000
IHC~~1:50~100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name CEP131
Synonyms AZI1, KIAA1118
Function Component of centriolar satellites contributing to build a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Acts also as a negative regulator of BBSome ciliary trafficking. Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail. Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability and centriole duplication in non-ciliogenic cells.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cytoplasm, cytoskeleton, cilium basal body. Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Colocalized with pericentriolar material protein PCM1 at centriolar satellites During spermiogenesis, becomes enriched with nephrocystin NPHP1 at the transition zone, a structure at the base of the ciliary axoneme important for regulating traffic into the ciliary compartment. Traffics towards and away from the centrosome/basal body and the transition zone of the ciliary axoneme in a microtubule-dependent manner. Localized at the Golgi-derived acrosome and the centrosome-containing head-tail coupling apparatus (HTCA) (By similarity). Ubiquitinated form is sequestred and colocalized with BBS4, CEP290, PCM1 and PCNT at centriolar satellites in proliferating cells. Colocalized with the pericentriolar material protein PCM1 at centrosome. Traffics towards and away from centriolar satellites and centrosome in a microtubule- and dynein-dependent manner in interphase cells Displaced from centriolar satellites but still remained associated with centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAPK signaling.
Research Areas

BACKGROUND

May play a role in spermatogenesis (By similarity).

REFERENCES

Kikuno R.,et al.DNA Res. 6:197-205(1999).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Andersen J.S.,et al.Nature 426:570-574(2003).
Olsen J.V.,et al.Cell 127:635-648(2006).
Beausoleil S.A.,et al.Nat. Biotechnol. 24:1285-1292(2006).

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