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CLASP1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CLASP1 Antibody AP50795
    Western blot analysis of lysate from Hela cell line,using CLASP1 Antibody(AP50795). AP50795 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • 2 - CLASP1 Antibody AP50795
    Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CLASP1 antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession Q7Z460
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 169,162,165,163 KDa
Additional info
Gene ID 23332
Other Names CLIP-associating protein 1, Cytoplasmic linker-associated protein 1, Multiple asters homolog 1, Protein Orbit homolog 1, hOrbit1, CLASP1, KIAA0622, MAST1
Dilution WB~~ 1:1000
IHC~~1:50~100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name CLASP1
Synonyms KIAA0622, MAST1
Function Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golgi network Note=Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2
Research Areas

BACKGROUND

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

REFERENCES

Maiato H.,et al.Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Akhmanova A.,et al.Cell 104:923-935(2001).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Ishikawa K.,et al.DNA Res. 5:169-176(1998).

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