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Tubulin α Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Tubulin α Antibody AP50786
    Western blot analysis of lysate from HepG2 cell line,using Tubulin α Antibody(AP50786). AP50786 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • 1 - Tubulin α Antibody AP50786
    Western blot analysis of extracts from 3T3 cells (Lane 2), colo cells (Lane 3) and HuvEc cells (Lane 4), using Tubulin α Antibody. The lane on the left is treated with synthesized peptide
  • 3 - Tubulin α Antibody AP50786
    Immunofluorescence analysis of NIH/3T3 cells, using Tubulin α antibody.
  • 2 - Tubulin α Antibody AP50786
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Tubulin α antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC
Primary Accession Q71U36
Other Accession P68363
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 50,46 KDa
Additional info
Gene ID 7846
Other Names Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, TUBA1A, TUBA3
Dilution WB~~ 1:1000
IF~~1:100
IHC~~1:50~100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name TUBA1A
Synonyms TUBA3
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Expressed at a high level in fetal brain.
Research Areas

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

REFERENCES

Hall J.L.,et al.Nucleic Acids Res. 13:207-223(1985).
Crabtree D.V.,et al.Bioorg. Med. Chem. 9:1967-1976(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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