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MED27 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MED27 Antibody AP50763
    Western blot analysis of lysate from Hela cell line,using MED27 Antibody(AP50763). AP50763 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q6P2C8
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 35,31 KDa
Additional info
Gene ID 9442
Other Names Mediator of RNA polymerase II transcription subunit 27, Cofactor required for Sp1 transcriptional activation subunit 8, CRSP complex subunit 8, Mediator complex subunit 27, P37 TRAP/SMCC/PC2 subunit, Transcriptional coactivator CRSP34, MED27, CRSP34 {ECO:0000312|EMBL:AAD127211}, CRSP8
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name MED27
Synonyms CRSP34 {ECO:0000312|EMBL:AAD12721.1}, CR
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Cellular Location Nucleus
Research Areas

BACKGROUND

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

REFERENCES

Humphray S.J.,et al.Nature 429:369-374(2004).
Ryu S.,et al.Nature 397:446-450(1999).
Malik S.,et al.Mol. Cell 5:753-760(2000).
Lin L.,et al.Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
Sato S.,et al.Mol. Cell 14:685-691(2004).

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