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Cytochrome P450 27A1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Cytochrome P450 27A1 Antibody AP50757
    Western blot analysis of lysate from human liver tissue lysate,using Cytochrome P450 27A1 Antibody(AP50757). AP50757 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q02318
Reactivity Human
Host Rabbit
Clonality polyclonal
Calculated MW 60 KDa
Additional info
Gene ID 1593
Other Names Sterol 26-hydroxylase, mitochondrial, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, Cytochrome P-450C27/25, Cytochrome P450 27, Sterol 27-hydroxylase, Vitamin D(3) 25-hydroxylase, CYP27A1, CYP27
Dilution WB~~1:500
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name CYP27A1
Synonyms CYP27
Function Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity.
Cellular Location Mitochondrion membrane.
Research Areas

BACKGROUND

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity.

REFERENCES

Cali J.J.,et al.J. Biol. Chem. 266:7774-7778(1991).
Guo Y.-D.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:8668-8672(1993).
Zhang H.T.,et al.Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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