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TF2H2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TF2H2 Antibody AP50741
    Western blot analysis of lysates from K562 cell line and human skeletal muscle tissue lysate(from left to right),using TF2H2 Antibody(AP50741). AP50741 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q13888
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 44 KDa
Additional info
Gene ID 2966;728340
Other Names General transcription factor IIH subunit 2, Basic transcription factor 2 44 kDa subunit, BTF2 p44, General transcription factor IIH polypeptide 2, TFIIH basal transcription factor complex p44 subunit, GTF2H2, BTF2P44
Dilution WB~~1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name GTF2H2
Synonyms BTF2P44
Function Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C- terminus is required for a successful escape of RNAP II form the promoter.
Cellular Location Nucleus.
Tissue Location Widely expressed, with higher expression in skeletal muscle
Research Areas

BACKGROUND

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C- terminus is required for a successful escape of RNAP II form the promoter.

REFERENCES

Humbert S.,et al.EMBO J. 13:2393-2398(1994).
Zhang Q.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
van der Steege G.,et al.Eur. J. Hum. Genet. 3:87-95(1995).
Chen Q.,et al.Genomics 48:121-127(1998).
Kershnar E.,et al.J. Biol. Chem. 273:34444-34453(1998).

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