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>   首页   >   产品   >   一抗   >   细胞生物学   >   CLK2 Antibody   

CLK2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CLK2 Antibody AP50695
    Western blot analysis of lysate from HUVEC cell line,using CLK2 Antibody(AP50695). AP50695 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P49760
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 60 18 KDa
Additional info
Gene ID 1196
Other Names Dual specificity protein kinase CLK2, CDC-like kinase 2, CLK2
Dilution WB~~1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name CLK2
Function Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Cellular Location Isoform 1: Nucleus. Nucleus speckle. Note=Inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle.
Tissue Location Endothelial cells.
Research Areas

BACKGROUND

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

REFERENCES

Hanes J.J.,et al.J. Mol. Biol. 244:665-672(1994).
Winfield S.L.,et al.Genome Res. 7:1020-1026(1997).
Gregory S.G.,et al.Nature 441:315-321(2006).
Lee K.,et al.J. Biol. Chem. 271:27299-27303(1996).
Duncan P.I.,et al.Exp. Cell Res. 241:300-308(1998).

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