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>   首页   >   产品   >   一抗   >   其他   >   RPA2 Antibody   

RPA2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RPA2 Antibody AP50624
    Western blot analysis of lysate from K562 cell line,using RPA2 Antibody(AP50624). AP50624 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9H9Y6
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 128,122 KDa
Additional info
Gene ID 84172
Other Names DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B
Dilution WB~~ 1:500
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name POLR1B
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).
Cellular Location Nucleus, nucleolus
Research Areas

BACKGROUND

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

REFERENCES

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Miller G.,et al.EMBO J. 20:1373-1382(2001).
Panov K.I.,et al.Mol. Cell. Biol. 26:5436-5448(2006).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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