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Cullin 2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Cullin 2 Antibody AP50189
    Western blot analysis of lysate from MDA-MB-231 cell line,using Cullin 2 Antibody(C0163). C0163 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
  • 3 - Cullin 2 Antibody AP50189
    Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody .
  • 2 - Cullin 2 Antibody AP50189
    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody .
  • 1 - Cullin 2 Antibody AP50189
    Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 Antibody. The lane on the left is treated with systhesized peptide.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC
Primary Accession Q13617
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 87,89 KDa
Additional info
Gene ID 8453
Other Names Cullin-2, CUL-2, CUL2
Dilution WB~~ 1:1000
IF~~1:100
IHC~~1:50-1:100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name CUL2
Function Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Research Areas

BACKGROUND

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

REFERENCES

Pause A.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:2156-2161(1997).
Wada H.,et al.Biochem. Biophys. Res. Commun. 257:100-105(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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