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>   首页   >   产品   >   一抗   >   精选抗体   >   BUB1B Antibody   

BUB1B Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - BUB1B Antibody AP50052
    Western blot analysis of lysates from Hela cell line ,using BUB1B Antibody(C10142). C10142 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O60566
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 120, 106, 121 KDa
Additional info
Gene ID 701
Other Names Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, hBUBR1, Mitotic checkpoint kinase MAD3L, Protein SSK1, BUB1B, BUBR1, MAD3L, SSK1
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name BUB1B
Synonyms BUBR1, MAD3L, SSK1
Function Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Cellular Location Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5
Tissue Location Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index
Research Areas

BACKGROUND

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

REFERENCES

Donadelli R.,et al.Biochem. Biophys. Res. Commun. 246:881-887(1998).
Taylor S.S.,et al.J. Cell Biol. 142:1-11(1998).
Chan G.K.T.,et al.J. Cell Biol. 143:49-63(1998).
Cahill D.P.,et al.Nature 392:300-303(1998).
Davenport J.W.,et al.Genomics 55:113-117(1999).

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