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>   首页   >   产品   >   一抗   >   癌症   >   Smad2 Antibody (Ab-465)   

Smad2 Antibody (Ab-465)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Smad2 Antibody (Ab-465) AP50015
    Western blot analysis of lysates from HepG2,NIH/3T3 cell line (from left to right),using Smad2 Antibody (Ab-465)(B9058). B9058 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q15796
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 52, 50 KDa
Additional info
Gene ID 4087
Other Names Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name SMAD2
Synonyms MADH2, MADR2
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1
Tissue Location Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta
Research Areas

BACKGROUND

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

REFERENCES

Riggins G.J.,et al.Nat. Genet. 13:347-349(1996).
Zhang Y.,et al.Nature 383:168-172(1996).
Eppert K.,et al.Cell 86:543-552(1996).
Liu F.,et al.Genes Dev. 11:3157-3167(1997).
Takenoshita S.,et al.Genomics 48:1-11(1998).

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