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>   首页   >   产品   >   一抗   >   癌症   >   NSE Antibody   

NSE Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NSE Antibody AP50004
    Western blot analysis of lysates from U-87 MG,SH-SY5Y cell line (from left to right),using NSE Antibody(C0280). C0280 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • 1 - NSE Antibody AP50004
    Western blot analysis of extracts from Hela cells (Lane 1), colo cells(Lane 2),HepG2 cells(Lane 3) and 293 cells (Lane 4), using NSE Antibody. The lane on the left is treated with synthesized peptide.
  • 3 - NSE Antibody AP50004
    Immunofluorescence analysis of A549 cells, using NSE antibody .
  • 2 - NSE Antibody AP50004
    Immunohistochemical analysis of paraffin-embedded human brain tissue using NSE antibody .
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC
Primary Accession P09104
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 47 KDa
Additional info
Gene ID 2026
Other Names Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Dilution WB~~ 1:1000
IF~~1:100
IHC~~1:50-1:100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name ENO2
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Cellular Location Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas

BACKGROUND

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

REFERENCES

McAleese S.M.,et al.Eur. J. Biochem. 178:413-417(1988).
van Obberghen E.,et al.J. Neurosci. Res. 19:450-456(1988).
Oliva D.,et al.Gene 79:355-360(1989).
Oliva D.,et al.Genomics 10:157-165(1991).
Ansari-Lari M.A.,et al.Genome Res. 7:268-280(1997).

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