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>   首页   >   产品   >   一抗   >   精选抗体   >   G蛋白偶联受体抗体(GPCR)   >   CCR7 Antibody (N-term)   

CCR7 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CCR7 Antibody (N-term) AP4998a
    All lanes : Anti-CCR7 Antibody (N-term) at 1:500-1:1000 dilution Lane 1: MDA-MB-231 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CCR7 Antibody (N-term) AP4998a
    Anti-CCR7 Antibody (Nterm) at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CCR7 Antibody (N-term) AP4998a
    CCR7 Antibody (N-term) (Cat. #AP4998a) western blot analysis in 293(lane 1),Ramos(lane 2),MDA-MB231(lane 3) cell line and mouse spleen tissue(lane 4) lysates (35ug/lane).This demonstrates the CCR7 antibody detected the CCR7 protein (arrow).
  • 3 - CCR7 Antibody (N-term) AP4998a
    Confocal immunofluorescent analysis of CCR7 Antibody (N-term)(Cat#AP4998a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 4 - CCR7 Antibody (N-term) AP4998a
    CCR7 Antibody (N-term) (Cat. #AP4998a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, FC, IHC-P, E
Primary Accession P32248
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1236
Other Names C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, EBV-induced G-protein coupled receptor 1, MIP-3 beta receptor, CD197, CCR7, CMKBR7, EBI1, EVI1
Target/Specificity This CCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-37 amino acids from the N-terminal region of human CCR7.
Dilution WB~~1:1000
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCR7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCR7
Synonyms CMKBR7, EBI1, EVI1
Function Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7
Research Areas

BACKGROUND

CCR7 is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor.

REFERENCES

Sun, J., et al. Cell. Mol. Immunol. 7(1):77-82(2010) Wu, W.L., et al. Eur. J. Immunol. 39(12):3413-3422(2009) Marcenaro, E., et al. Blood 114(19):4108-4116(2009)

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