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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-mouse ERBB2(Y1197) Antibody   

Phospho-mouse ERBB2(Y1197) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-mouse ERBB2(Y1197) Antibody AP3781o
    Dot blot analysis of Phospho-mouse ERBB2-Y1197 Antibody Phospho-specific Pab (Cat. #AP3781o) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession P70424
Other Accession NP_001003817.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 138579 Da
Additional info
Gene ID 13866
Other Names Receptor tyrosine-protein kinase erbB-2, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, CD340, Erbb2, Kiaa3023, Neu
Target/Specificity This mouse ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1197 of mouse ERBB2.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-mouse ERBB2(Y1197) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Erbb2
Synonyms Kiaa3023, Neu
Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus
Tissue Location Expressed predominantly in uterine epithelial cells. In the muscle, expression localizes to the synaptic sites of muscle fibers
Research Areas

BACKGROUND

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

REFERENCES

Cabodi, S., et al. FASEB J. 24(10):3796-3808(2010)
Johnson, E., et al. J. Biol. Chem. 285(38):29491-29501(2010)
Huck, L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15559-15564(2010)
Chuang, T.D., et al. J. Biol. Chem. 285(31):23598-23606(2010)
Simeone, L., et al. J. Neurosci. 30(19):6620-6634(2010)

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