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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-BAD(Y76) Antibody   

Phospho-BAD(Y76) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-BAD(Y76) Antibody AP3777j
    Dot blot analysis of Phospho-BAD-Y76 Antibody Phospho-specific Pab (Cat. #AP3777j) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession Q92934
Other Accession NP_004313.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18392 Da
Additional info
Gene ID 572
Other Names Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BAD, BBC6, BCL2L8
Target/Specificity This BAD Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y76 of human BAD.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-BAD(Y76) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BAD
Synonyms BBC6, BCL2L8
Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Cellular Location Mitochondrion outer membrane. Cytoplasm {ECO:0000250|UniProtKB:Q61337}. Note=Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm. {ECO:0000250|UniProtKB:Q61337}
Tissue Location Expressed in a wide variety of tissues.
Research Areas

BACKGROUND

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq].

REFERENCES

Chen, B., et al. Am. J. Physiol., Cell Physiol. 299 (5), C968-C976 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Galmiche, A., et al. Mol. Cancer Res. 8(8):1116-1125(2010)
Cerioni, L., et al. Methods Mol. Biol. 648, 291-301 (2010) :
Yu, B., et al. J. Exp. Clin. Cancer Res. 29, 107 (2010) :

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