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Phospho-Belcin 1 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-Belcin 1 Antibody AP3765a
    Dot blot analysis of Beclin 1 phospho antibody Phospho-specific Pab (Cat. #AP3765a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession Q14457
Other Accession Q6GP52, Q91XJ1, Q4A1L5, O88597, Q5ZKS6, Q4A1L4, NP_003757.1
Reactivity Human
Predicted Bovine, Chicken, Mouse, Pig, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51896 Da
Additional info
Gene ID 8678
Other Names Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Target/Specificity This Belcin 1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T119 of human Belcin 1.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Belcin 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BECN1
Synonyms GT197
Function Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19713971, ECO:0000269|PubMed:21364619}. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome {ECO:0000250|UniProtKB:O88597}. Cytoplasmic vesicle, autophagosome. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19050071} Beclin-1-C 37 kDa: Mitochondrion {ECO:0000250|UniProtKB:O88597}
Tissue Location Ubiquitous.
Research Areas
Lipin-1 determines lung cancer cell survival and chemotherapy sensitivity by regulation of endoplasmic reticulum homeostasis and autophagy.
Author : Fan X1,Weng Y1,Bai Y1,Wang Z2,Wang S1,3,Zhu J1,Zhang F1.
Cancer Med. 2018 Jun;7(6):2541-2554. doi: 10.1002/cam4.1483. Epub 2018 Apr 16.
29659171
Regulation of Beclin 1 Protein Phosphorylation and Autophagy by Protein Phosphatase 2A (PP2A) and Death-associated Protein Kinase 3 (DAPK3).
Author : Fujiwara N1, Usui T2, Ohama T3, Sato K1.
J Biol Chem. 2016 May 13;291(20):10858-66. doi: 10.1074/jbc.M115.704908. Epub 2016 Mar 18.
26994142

BACKGROUND

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM].

REFERENCES

Koukourakis, M.I., et al. Br. J. Cancer 103(8):1209-1214(2010) Jaeger, P.A., et al. Arch. Neurol. 67(10):1181-1184(2010) Metzger, S., et al. Hum. Genet. 128(4):453-459(2010) Oberstein, A., et al. J. Biol. Chem. 282(17):13123-13132(2007) Furuya, N., et al. Autophagy 1(1):46-52(2005)

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