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Phospho-PDHE1A(S232) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-PDHE1A(S232) Antibody AP3758A
    Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab (Cat. #AP3758a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession P08559
Other Accession P26284, P29804, P35486, Q8HXW9, A7MB35, NP_000275.1
Reactivity Human
Predicted Bovine, Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43296 Da
Additional info
Gene ID 5160
Other Names Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1, PHE1A
Target/Specificity This PDHE1A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S232 of human PDHE1A.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-PDHE1A(S232) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDHA1
Synonyms PHE1A
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Tissue Location Ubiquitous.
Research Areas
PINK1 Is a Negative Regulator of Growth and the Warburg Effect in Glioblastoma.
Author : Agnihotri S1, Golbourn B2, Huang X3, Remke M2, Younger S4, Cairns RA5, Chalil A2, Smith CA2, Krumholtz SL2, Mackenzie D2, Rakopoulos P2 Cancer Res. 2016 Jun 20. [Epub ahead of print]
27325644
JX06 Selectively Inhibits Pyruvate Dehydrogenase Kinase PDK1 by a Covalent Cysteine Modification.
Author : Sun W1,Xie Z1,Liu Y2,Zhao D3,Wu Z4,Zhang D1,Lv H1,Tang S1,Jin N1,Jiang H3,Tan M4,Ding J1,Luo C5,Li J6,Huang M7,Geng M7.
Cancer Res. 2015 Nov 15;75(22):4923-36. doi: 10.1158/0008-5472.CAN-15-1023. Epub 2015 Oct 19.
26483203
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