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Phospho-SHP2(Y584) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Phospho-SHP2(Y584) Antibody AP3716a
    Western blot analysis of lysate from NIH-3T3 cells(from left to right),untreated or treated with PDGF at 50ng/ml,using Phospho-SHP2(Y584) Antibody (Cat. #AP3716a) or SHP2 Antibody.Lysate at 15ug per lane.AP3716a was diluted at 1:2000 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
  • 6 - Phospho-SHP2(Y584) Antibody AP3716a
    Dot blot analysis of anti-Phospho-SHP2-pY584 Phospho-specific Pab (Cat. #AP3716a ) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, WB, E
Primary Accession Q06124
Other Accession P41499, P35235
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68436 Da
Additional info
Gene ID 5781
Other Names Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2
Target/Specificity This SHP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y584 of human SHP2.
Dilution WB~~1:2000
DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-SHP2(Y584) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPN11
Synonyms PTP2C, SHPTP2
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).
Cellular Location Cytoplasm. Nucleus
Tissue Location Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Research Areas

BACKGROUND

SHP2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

REFERENCES

Carver, K.C., et al. J. Biol. Chem. 285(11):8003-8012(2010)
Pierpont, E.I., et al. Am. J. Med. Genet. A 152A (3), 591-600 (2010)
Rani, D.S., et al. Mitochondrion 10(2):166-173(2010)
Bakken, T., et al. Virology 397(2):379-388(2010)

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