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Phospho-RUNX2(S465) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-RUNX2(S465) Antibody AP3559a
    Dot blot analysis of anti-Phospho-RUNX2-pS465 Antibody (Cat.#AP3559a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
  • 产品详情
  • 文献引用 : 9
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession Q13950
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 860
Other Names Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, CBF-alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirus enhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, RUNX2, AML3, CBFA1, OSF2, PEBP2A
Target/Specificity This RUNX2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S465 of human RUNX2.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-RUNX2(S465) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUNX2
Synonyms AML3, CBFA1, OSF2, PEBP2A
Function Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
Cellular Location Nucleus.
Tissue Location Specifically expressed in osteoblasts.
Research Areas
Material-driven fibronectin assembly for high-efficiency presentation of growth factors.
Author : Llopis-Hernández V1, Cantini M1, González-García C1, Cheng ZA1, Yang J2, Tsimbouri PM2, García AJ3, Dalby MJ2, Salmerón-Sánchez M1.
Sci Adv. 2016 Aug 26;2(8):e1600188. doi: 10.1126/sciadv.1600188. eCollection 2016.
27574702
The natural compound codonolactone attenuates TGF-β1-mediated epithelial-to-mesenchymal transition and motility of breast cancer cells.
Author : Fu J1, Ke X1, Tan S1, Liu T1, Wang S1, Ma J1, Lu H2.
Oncol Rep. 2015 Nov 4. doi: 10.3892/or.2015.4394. [Epub ahead of print]
26549400
The natural compound codonolactone impairs tumor induced angiogenesis by downregulating BMP signaling in endothelial cells.
Author : Wang S1, Cai R1, Ma J1, Liu T1, Ke X1, Lu H2, Fu J3.
Phytomedicine. 2015 Oct 15;22(11):1017-26. doi: 10.1016/j.phymed.2015.07.009. Epub 2015 Aug 19.
26407944
In vitro inhibitory effects of terpenoids from Chloranthus multistachys on epithelial-mesenchymal transition via down-regulation of Runx2 activation in human breast cancer.
Author : Fu J1, Wang S1, Lu H2, Ma J1, Ke X1, Liu T1, Luo Y3.
Phytomedicine. 2015 Jan 15;22(1):165-72. doi: 10.1016/j.phymed.2014.11.010. Epub 2014 Nov 27.
25636886
Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2.
Author : Wang W1, Chen B1, Zou R2, Tu X1, Tan S1, Lu H3, Liu Z1, Fu J1.
Int J Oncol. 2014 Sep 4. doi: 10.3892/ijo.2014.2643. [Epub ahead of print]
25190326
A genomics approach in determining nanotopographical effects on MSC phenotype.
Author : Tsimbouri PM, Murawski K, Hamilton G, Herzyk P, Oreffo RO, Gadegaard N, Dalby MJ.
Biomaterials. 2013 Mar;34(9):2177-84. doi: 10.1016/j.biomaterials.2012.12.019. Epub 2013 Jan 9.
23312853
Using nanotopography and metabolomics to identify biochemical effectors of multipotency.
Author : Tsimbouri PM, McMurray RJ, Burgess KV, Alakpa EV, Reynolds PM, Murawski K, Kingham E, Oreffo RO, Gadegaard N, Dalby MJ.
ACS Nano. 2012 Nov 27;6(11):10239-49. doi: 10.1021/nn304046m. Epub 2012 Oct 24.
23072705
Skeletal stem cell physiology on functionally distinct titania nanotopographies.
Author : McNamara LE, Sjöström T, Burgess KE, Kim JJ, Liu E, Gordonov S, Moghe PV, Meek RM, Oreffo RO, Su B, Dalby MJ.
Biomaterials. 2011 Oct;32(30):7403-10. doi: 10.1016/j.biomaterials.2011.06.063. Epub 2011 Aug 4.
21820172
Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells.
Author : Tetsunaga T, Nishida K, Furumatsu T, Naruse K, Hirohata S, Yoshida A, Saito T, Ozaki T.
Osteoarthritis Cartilage. 2011 Feb;19(2):222-32. doi: 10.1016/j.joca.2010.11.004. Epub 2010 Nov 19.
21094261

BACKGROUND

Runx2 is a member of the RUNX family of transcription factors. It is a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in the Runx2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD).

REFERENCES

Rich,J.T., Biochem. Biophys. Res. Commun. 372 (1), 230-235 (2008) Ermakov,S., Ann. Hum. Genet. 72 (PT 4), 510-518 (2008) Endo,T., J. Clin. Endocrinol. Metab. 93 (6), 2409-2412 (2008)

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