|Application ||DB, E|
|Other Names||Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, Nck-interacting kinase, MAP4K4, HGK, KIAA0687, NIK|
|Target/Specificity||This MAP4K4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S801 of human MAP4K4.|
|Format||Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-MAP4K4(S801) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HGK, KIAA0687, NIK|
|Function||Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.|
|Tissue Location||Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.|
M4K4, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.
Wright, J.H., et al., Mol. Cell. Biol. 23(6):2068-2082 (2003).
Yao, Z., et al., J. Biol. Chem. 274(4):2118-2125 (1999).
Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998).