|Application ||DB, E|
|Other Accession||Q63768, Q64010, Q96GA9|
|Calculated MW||33831 Da|
|Other Names||Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK|
|Target/Specificity||This CRK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S41 of human CRK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-CRK(S41) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.|
Provided below are standard protocols that you may find useful for product applications.
CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.
Bougneres, L., et al., J. Cell Biol. 166(2):225-235 (2004).
Stoletov, K.V., et al., Exp. Cell Res. 295(1):258-268 (2004).
Miller, C.T., et al., Oncogene 22(39):7950-7957 (2003).
Sun, J., et al., J. Biol. Chem. 278(35):32794-32800 (2003).
Zhang, X.A., et al., J. Biol. Chem. 278(29):27319-27328 (2003).