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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-SMAD4(T277) Antibody   

Phospho-SMAD4(T277) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-SMAD4(T277) Antibody AP3251a
    Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-β(100ng/ml, 30min), using Phospho-SMAD4(T277) Antibody(upper) or Beta-actin (lower).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q13485
Other Accession O70437, P97471
Reactivity Human, Mouse
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4089
Other Names Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
Target/Specificity This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-SMAD4(T277) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD4
Synonyms DPC4, MADH4
Function In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed:25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD (PubMed:15799969). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236)
Research Areas
Increased Retinal Expression of the Pro-Angiogenic Receptor GPR91 via BMP6 in a Mouse Model of Juvenile Hemochromatosis.
Author : Arjunan P1, Gnanaprakasam JP2, Ananth S2, Romej MA2, Rajalakshmi VK2, Prasad PD2, Martin PM2, Gurusamy M2, Thangaraju M2, Bhutia YD3, Ganapathy V
Invest Ophthalmol Vis Sci. 2016 Apr 1;57(4):1612-9. doi: 10.1167/iovs.15-17437.
27046124

BACKGROUND

Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

REFERENCES

Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004).
Horvath, L.G., et al., Prostate 59(3):234-242 (2004).
Li, L., et al., Mol. Cell. Biol. 24(2):856-864 (2004).
Wan, M., et al., J. Biol. Chem. 279(15):14484-14487 (2004).
Maru, D., et al., Oncogene 23(3):859-864 (2004).

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