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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-NFKB(S536) Antibody   

Phospho-NFKB(S536) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-NFKB(S536) Antibody AP3178a
    Western blot analysis of lysates from Hela cell line, untreated or treated with TNF-α, 20 ng/ml, using Phospho-NFKBp65(S536) Antibody (Cat. #AP3176a)(upper) or Beta-actin (lower).
  • 产品详情
  • 文献引用 : 3
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  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q04206
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 5970
Other Names Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
Target/Specificity This NFKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NFKB.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-NFKB(S536) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RELA
Synonyms NFKB3
Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
Cellular Location Nucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity) Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation.
Research Areas
Nanoformulated paclitaxel and AZD9291 synergistically eradicate non-small-cell lung cancers in vivo.
Author : Wang XS1,Zhang L1,Li X2,Kong DJ1,Hu XC1,Ding XZ1,Yang JQ1,Zhao MQ1,He Y2,Lam KS2,Gao SG1,Lin TY3,Li Y2.
Nanomedicine (Lond). 2018 Jun 6. doi: 10.2217/nnm-2017-0355. [Epub ahead of print]
29874151
miR-221/222 promote cancer stem-like cell properties and tumor growth of breast cancer via targeting PTEN and sustained Akt/NF-κB/COX-2 activation.
Author : Li B1,Lu Y2,Yu L3,Han X3,Wang H4,Mao J3,Shen J2,Wang B3,Tang J3,Li C5,Song B6.
Chem Biol Interact. 2017 Aug 24;277:33-42. doi: 10.1016/j.cbi.2017.08.014. [Epub ahead of print]
28844858
Activation of NF-{kappa}B by TMPRSS2/ERG Fusion Isoforms through Toll-Like Receptor-4.
Author : Wang J, Cai Y, Shao LJ, Siddiqui J, Palanisamy N, Li R, Ren C, Ayala G, Ittmann M.
Cancer Res. 2011 Feb 15;71(4):1325-33. doi: 10.1158/0008-5472.CAN-10-2210. Epub 2010 Dec 17.
21169414

BACKGROUND

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

REFERENCES

Zamora, M., et al., J. Biol. Chem. 279(37):38415-38423 (2004). Jeong, S.J., et al., Blood 104(5):1490-1497 (2004). Bohuslav, J., et al., J. Biol. Chem. 279(25):26115-26125 (2004). Yeh, P.Y., et al., J. Biol. Chem. 279(25):26143-26148 (2004). Jang, H.D., et al., J. Biol. Chem. 279(23):24873-24880 (2004).

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