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Phospho-CREB(S133) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 59 - Phospho-CREB(S133) Antibody AP3077a
    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue using AP3077a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - Phospho-CREB(S133) Antibody AP3077a
    Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using AP3077a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - Phospho-CREB(S133) Antibody AP3077a
    Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-CREB(S133) Antibody(upper) or tubulin(lower).
  • 1 - Phospho-CREB(S133) Antibody AP3077a
    Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-CREB(S133) Antibody(upper) or tubulin(lower).
  • 1 - Phospho-CREB(S133) Antibody AP3077a
    Western blot analysis of lysates from Hela cell line, untreated or treated with 20% FBS + 100nM Calyculin A, using Phospho-CREB(S133) Antibody(upper) or tubulin(lower).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, IF, WB, E
Primary Accession P16220
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36688 Da
Additional info
Gene ID 1385
Other Names Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Target/Specificity This CREB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB.
Dilution IHC-P-Leica~~1:500
WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CREB(S133) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CREB1
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083}
Research Areas
Transgenic songbirds with suppressed or enhanced activity of CREB transcription factor.
Author : Abe K1, Matsui S2, Watanabe D3.
Proc Natl Acad Sci U S A. 2015 Jun 5. pii: 201413484. [Epub ahead of print]
26048905

BACKGROUND

CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.

REFERENCES

Samten, B., et al., J. Immunol. 174(10):6357-6363 (2005).
Zhang, X., et al., Proc. Natl. Acad. Sci. U.S.A. 102(12):4459-4464 (2005).
Cho, K.N., et al., J. Biol. Chem. 280(8):6676-6681 (2005).
Bailey, J., et al., J. Mol. Endocrinol. 34(1):1-17 (2005).
Perry, C., et al., Neoplasia 6(3):279-286 (2004).

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