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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-CDC25A(S178) Antibody   

Phospho-CDC25A(S178) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-CDC25A(S178) Antibody AP3046a
    The anti-Phospho-CDC25A-S178 Pab (Cat. #AP3046a) is used in Western blot to detect Phospho-CDC25A-S177 in mouse kidney tissue lysate
  • 14 - Phospho-CDC25A(S178) Antibody AP3046a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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  • 文献引用 : 10
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P30304
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 993
Other Names M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A
Target/Specificity This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S177 of human CDC25A.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CDC25A(S178) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25A
Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Research Areas
Mangrove dolabrane-type of diterpenes tagalsins suppresses tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest.
Author : Neumann J1, Yang Y2, Köhler R1, Giaisi M1, Witzens-Harig M3, Liu D2, Krammer PH1, Lin W2, Li-Weber M1.
Int J Cancer. 2015 Jun 9. doi: 10.1002/ijc.29629. [Epub ahead of print]
26061604
A eudesmane-type sesquiterpene isolated from Pluchea odorata (L.) Cass. combats three hallmarks of cancer cells: Unrestricted proliferation, escape from apoptosis and early metastatic outgrowth in vitro.
Author : Blaschke M1, McKinnon R2, Nguyen CH3, Holzner S4, Zehl M5, Atanasov AG2, Schelch K6, Krieger S4, Diaz R7, Frisch R7, Feistel B8, Jäger
Mutat Res. 2015 May 5;777:79-90. doi: 10.1016/j.mrfmmm.2015.04.011. [Epub ahead of print]
25989051
Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells.
Author : Giessrigl B, Krieger S, Rosner M, Huttary N, Saiko P, Alami M, Messaoudi S, Peyrat JF, Maciuk A, Gollinger M, Kopf S, Kazlauskas E, Mazal P, Szekeres T, Hengstschläger M, Matulis D, Jäger W, Krupitza G.
Hum Mol Genet. 2012 Nov 1;21(21):4615-27. doi: 10.1093/hmg/dds303. Epub 2012 Jul 25.
22843495
Methanol extract of the ethnopharmaceutical remedy Smilax spinosa exhibits anti-neoplastic activity.
Author : Seelinger M, Popescu R, Giessrigl B, Jarukamjorn K, Unger C, Wallnöfer B, Fritzer-Szekeres M, Szekeres T, Diaz R, Jäger W, Frisch R, Kopp B, Krupitza G.
Int J Oncol. 2012 Sep;41(3):1164-72. doi: 10.3892/ijo.2012.1538. Epub 2012 Jun 29.
22752086
An apolar extract of Critonia morifolia inhibits c-Myc, cyclin D1, Cdc25A, Cdc25B, Cdc25C and Akt and induces apoptosis.
Author : Unger C, Popescu R, Giessrigl B, Rarova L, Herbacek I, Seelinger M, Diaz R, Wallnöfer B, Fritzer-Szekeres M, Szekeres T, Frisch R, Doležal K, Strnad M, De Martin R, Grusch M, Kopp B, Krupitza G.
Int J Oncol. 2012 Jun;40(6):2131-9. doi: 10.3892/ijo.2012.1412. Epub 2012 Mar 23.
22446629
A novel N-hydroxy-N'-aminoguanidine derivative inhibits ribonucleotide reductase activity: Effects in human HL-60 promyelocytic leukemia cells and synergism with arabinofuranosylcytosine (Ara-C).
Author : Saiko P, Graser G, Giessrigl B, Lackner A, Grusch M, Krupitza G, Basu A, Sinha BN, Jayaprakash V, Jaeger W, Fritzer-Szekeres M, Szekeres T.
Biochem Pharmacol. 2011 Jan 1;81(1):50-9. doi: 10.1016/j.bcp.2010.09.006. Epub 2010 Sep 16.
20849828
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
Author : Vo NT1, Madlener S, Bago-Horvath Z, Herbacek I, Stark N, Gridling M, Probst P, Giessrigl B, Bauer S, Vonach C, Saiko P, Grusch M, Szekeres T, Fritzer-Szekeres M, Jäger W, Krupitza G, Soleiman A.
Carcinogenesis. 2010 Dec;31(12):2074-81. doi: 10.1093/carcin/bgp199. Epub 2009 Aug 20.
19696164
Berberine and a Berberis lycium extract inactivate Cdc25A and induce alpha-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis.
Author : Khan M, Giessrigl B, Vonach C, Madlener S, Prinz S, Herbaceck I, Hölzl C, Bauer S, Viola K, Mikulits W, Quereshi RA, Knasmüller S, Grusch M, Kopp B, Krupitza G.
Mutat Res. 2010 Jan 5;683(1-2):123-30. doi: 10.1016/j.mrfmmm.2009.11.001.
19909759
Short 42 degrees C heat shock induces phosphorylation and degradation of Cdc25A which depends on p38MAPK, Chk2 and 14.3.3.
Author : Madlener S, Rosner M, Krieger S, Giessrigl B, Gridling M, Vo TP, Leisser C, Lackner A, Raab I, Grusch M, Hengstschläger M, Dolznig H, Krupitza G.
Hum Mol Genet. 2009 Jun 1;18(11):1990-2000. doi: 10.1093/hmg/ddp123. Epub 2009 Mar 16.
19289404
Carboxyl-terminal peptides as probes for Escherichia coli ribonucleotide reductase subunit interaction: kinetic analysis of inhibition studies.
Author : Climent I, Sjöberg BM, Huang CY.
Biochemistry. 1991 May 28;30(21):5164-71.
2036382

BACKGROUND

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). Nemoto, K., et al., Prostate 58(1):95-102 (2004). Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003). Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003). Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).

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