注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-CDC25A(S124) Antibody   

Phospho-CDC25A(S124) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-CDC25A(S124) Antibody AP3045a
    Western blot analysis of lysates from 293 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088102(Cat. #AP3045A)(upper) or Beta-actin (lower).
  • 1 - Phospho-CDC25A(S124) Antibody AP3045a
    Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088101(Cat. #AP3045A)(upper) or Beta-actin (lower).
  • 1 - Phospho-CDC25A(S124) Antibody AP3045a
    Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459085101(Cat. #AP3045A)(upper) or Beta-actin (lower).
  • 1 - Phospho-CDC25A(S124) Antibody AP3045a
    The anti-phospho CDC25A S124 Pab (Cat. #AP3045a) is used in Western blot to detect CDC25A in mouse liver tissue lysate
  • 14 - Phospho-CDC25A(S124) Antibody AP3045a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 产品详情
  • 文献引用 : 2
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P30304
Other Accession P48965
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 993
Other Names M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A
Target/Specificity This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human CDC25A.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CDC25A(S124) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25A
Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Research Areas
CHEK2 genomic and proteomic analyses reveal genetic inactivation or endogenous activation across the 60 cell lines of the US National Cancer Institute.
Author : Zoppoli G, Solier S, Reinhold WC, Liu H, Connelly JW Jr, Monks A, Shoemaker RH, Abaan OD, Davis SR, Meltzer PS, Doroshow JH, Pommier Y.
Oncogene. 2012 Jan 26;31(4):403-18. doi: 10.1038/onc.2011.283. Epub 2011 Jul 18.
21765476
Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways.
Author : Solier S, Sordet O, Kohn KW, Pommier Y.
Mol Cell Biol. 2009 Jan;29(1):68-82. doi: 10.1128/MCB.00581-08. Epub 2008 Oct 27.
18955500

BACKGROUND

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). Nemoto, K., et al., Prostate 58(1):95-102 (2004). Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003). Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003). Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价