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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-H3(S10) Antibody   

Phospho-H3(S10) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-H3(S10) Antibody AP3003a
    Western blot analysis of anti-Phospho-H3-pS10 Pab (Cat. #AP3003a) in CEM cell line lysate (35ug/lane). Phospho-H3-pS10(arrow) was detected using the purified Pab.
  • 14 - Phospho-H3(S10) Antibody AP3003a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P68431
Other Accession P61830, P02299, P08898, P02302, P02301, Q6NXT2, A5PK61, Q6PI79, P84245, P84246, Q71LE2, P84244, P84243, P84249, Q6PI20, P84247, Q5E9F8, Q27532, Q9U281, Q10453, P84233, P84228, Q71DI3, Q4QRF4, P84229, P84227, Q6LED0, P68433, P68432, Q16695, Q71DJ3
Reactivity Human
Predicted Bovine, Mouse, Rat, Chicken, Zebrafish, Xenopus, C.Elegans, Drosophila, Pig, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 15404 Da
Additional info
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Other Names Histone H31, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA
Target/Specificity This H3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human H3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-H3(S10) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H3A
Synonyms H3FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas

BACKGROUND

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The gene for this protein is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. The gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

REFERENCES

Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003).
Deng, L., et al., Virology 289(2):312-326 (2001).
Deng, L., et al., Virology 277(2):278-295 (2000).
El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998).
Albig, W., et al., Hum. Genet. 101(3):284-294 (1997).

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