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YWHAG Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - YWHAG Antibody (Center) AP2939c
    Western blot analysis of lysate from Hela cell line, using YWHAG Antibody (Center)(Cat. #AP2939c). AP2939c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 1 - YWHAG Antibody (Center) AP2939c
    Western blot analysis of YWHAG Antibody (Center) (Cat. #AP2939c) in mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab.
  • 14 - YWHAG Antibody (Center) AP2939c
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with YWHAG Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - YWHAG Antibody (Center) AP2939c
    Flow cytometric analysis of widr cells using YWHAG Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 1 - YWHAG Antibody (Center) AP2939c
    Western blot analysis of YWHAG (arrow) using rabbit polyclonal YWHAG Antibody (Center) (Cat. #AP2939c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAG gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P61981
Other Accession Q6NRY9, Q6PCG0, P61983, P61982, Q5F3W6, P68252
Reactivity Human, Mouse
Predicted Bovine, Chicken, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28303 Da
Additional info
Gene ID 7532
Other Names 14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma, N-terminally processed, YWHAG
Target/Specificity This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human YWHAG.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsYWHAG Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YWHAG
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in brain, skeletal muscle, and heart.
Research Areas

BACKGROUND

YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

REFERENCES

Jagemann,L.R., et.al., J. Biol. Chem. 283 (25), 17450-17462 (2008)

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