|Application ||WB, IHC-P, FC, E|
|Calculated MW||56782 Da|
|Other Names||Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3, ERP57, ERP60, GRP58|
|Target/Specificity||This PDIA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human PDIA3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDIA3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ERP57, ERP60, GRP58|
|Cellular Location||Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).|
|Tissue Location||Detected in the flagellum and head region of spermatozoa (at protein level).|
Provided below are standard protocols that you may find useful for product applications.
PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Vigneron,N., et.al., Eur. J. Immunol. 39 (9), 2371-2376 (2009)
Xu,D.,et.al., Am. J. Physiol. Lung Cell Mol. Physiol. 297 (1), L44-L51 (2009)