|Application ||IF, WB, E|
|Calculated MW||94103 Da|
|Other Names||Exonuclease 1, hExo1, 31--, Exonuclease I, hExoI, EXO1, EXOI, HEX1|
|Target/Specificity||This EXO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EXO1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EXO1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.|
|Cellular Location||Nucleus Note=Colocalizes with PCNA to discrete nuclear foci in S-phase|
|Tissue Location||Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach|
Provided below are standard protocols that you may find useful for product applications.
EXO1 is a protein with 5' to 3' exonuclease activity as well as an RNase H activity. This protein is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination.
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