注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   GADD45A Antibody (C-term)   

GADD45A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - GADD45A Antibody (C-term) AP2785b
    Western blot analysis of GADD45A Antibody (C-term) (Cat. #AP2785b) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.
  • 4 - GADD45A Antibody (C-term) AP2785b
    GADD45A Antibody (C-term) (Cat. #AP2785b) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 1 - GADD45A Antibody (C-term) AP2785b
    Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (C-term) (Cat. #AP2785b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.
  • 产品详情
  • 文献引用 : 1
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P24522
Other Accession P48317, P48316, P24523, Q3ZBN6
Reactivity Human
Predicted Bovine, Hamster, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18336 Da
Additional info
Gene ID 1647
Other Names Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45
Target/Specificity This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the C-terminal region of human GADD45A.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGADD45A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GADD45A
Synonyms DDIT1, GADD45
Function In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
Cellular Location Nucleus.
Research Areas
Significance of S100P as a biomarker in diagnosis, prognosis and therapy of opisthorchiasis-associated cholangiocarcinoma.
Author : Wu Z1, Boonmars T2,3, Nagano I1, Boonjaraspinyo S4, Srinontong P1, Ratasuwan P5, Narong K3,6, Nielsen PS7, Maekawa Y1.
Int J Cancer. 2015 Aug 8. doi: 10.1002/ijc.29721. [Epub ahead of print]
26312563

BACKGROUND

GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.

REFERENCES

Li,L.S., J. Biol. Chem. 283 (31), 21394-21403 (2008)
Al-Romaih,K., Neoplasia 10 (5), 471-480 (2008)
Zhu,Q.S., Cancer Res. 68 (8), 2895-2903 (2008)
Kearsey,J.M., Oncogene 11 (9), 1675-1683 (1995)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价