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>   首页   >   产品   >   一抗   >   神经科学   >   DCX Antibody (S128)   

DCX Antibody (S128)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - DCX Antibody (S128) AP2768a
    Western blot analysis of lysate from human brain tissue lysate, using Phospho-DCX-S128. ctrl(Cat. #AP2768a). AP2768a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • 14 - DCX Antibody (S128) AP2768a
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DCX Antibody (S128) (Cat.#AP2768a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession O43602
Other Accession Q9ESI7, O88809
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40574 Da
Additional info
Gene ID 1641
Other Names Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX, DBCN, LISX
Target/Specificity This DCX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-137 amino acids from human DCX.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDCX Antibody (S128) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DCX
Synonyms DBCN, LISX
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Cellular Location Cytoplasm. Cell projection {ECO:0000250|UniProtKB:Q9ESI7}. Note=Localizes at neurite tips {ECO:0000250|UniProtKB:Q9ESI7}
Tissue Location Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas
Research Areas

BACKGROUND

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.

REFERENCES

Leger,P.L., Neurogenetics (2008)
Zhang,Y., Biochem. Biophys. Res. Commun. 363 (3), 694-700 (2007)

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