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TAL1 Antibody (T90)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - TAL1 Antibody (T90) AP2754A
    Immunohistochemical analysis of paraffin-embedded Human marrow tissue using AP2754A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - TAL1 Antibody (T90) AP2754A
    All lanes : Anti-TAL1 Antibody (T90) at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession P17542
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34271 Da
Additional info
Gene ID 6886
Other Names T-cell acute lymphocytic leukemia protein 1, TAL-1, Class A basic helix-loop-helix protein 17, bHLHa17, Stem cell protein, T-cell leukemia/lymphoma protein 5, TAL1, BHLHA17, SCL, TCL5
Target/Specificity This TAL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human TAL1.
Dilution IHC-P-Leica~~1:500
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAL1 Antibody (T90) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAL1
Synonyms BHLHA17, SCL, TCL5
Function Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}
Tissue Location Leukemic stem cell.
Research Areas

BACKGROUND

TAL1 is implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. It serves as a positive regulator of erythroid differentiation.

REFERENCES

Kassouf,M.T., Blood 112 (4), 1056-1067 (2008)
Terme,J.M., J. Virol. 82 (16), 7913-7922 (2008)
Brunet de la Grange,P., Stem Cells 26 (6), 1658-1662 (2008)

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