- 文献引用 : 4
|Application ||WB, E|
|Other Names||Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67|
|Target/Specificity||This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LGR5/GPR49 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).|
|Tissue Location||Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver|
Author : Usui T1,Sakurai M2,Enjoji S3,Kawasaki H3,Umata K3,Ohama T3,Fujiwara N3,Yabe R3,Tsuji S3,Yamawaki H4,Hazama S5,Takenouchi H6,Nakajima M6,Tsunedomi R6,Suzuki N6,Nagano H6,Sato K3.
Stem Cells Int. 2016;2016:7053872. doi: 10.1155/2016/7053872. Epub 2016 Dec 29.
Author : Jardé T1, Kass L2, Staples M3, Lescesen H2, Carne P4, Oliva K4, McMurrick PJ4, Abud HE2.
PLoS One. 2015 Sep 14;10(9):e0138336. doi: 10.1371/journal.pone.0138336. eCollection 2015.
Author : Fender AW1, Nutter JM, Bertrand FE, Sigounas G.
J Cell Biochem. 2015 Apr 25. doi: 10.1002/jcb.25196. [Epub ahead of print]
Author : Kanwar SS, Yu Y, Nautiyal J, Patel BB, Majumdar AP.
Mol Cancer. 2010 Aug 6;9:212. doi: 10.1186/1476-4598-9-212.
Provided below are standard protocols that you may find useful for product applications.
LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.
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McClanahan,T., Cancer Biol. Ther. 5 (4), 419-426 (2006)
Yamamoto,Y., Hepatology 37 (3), 528-533 (2003)
Hsu,S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000)